:root {
            --primary-color: #2c7da0;
            --secondary-color: #a9d6e5;
            --accent-color: #01497c;
            --light-gray: #f8f9fa;
            --border-color: #eaeaea;
            --text-color: #333;
            --text-muted: #6c757d;
            --success-color: #28a745;
            --warning-color: #ffc107;
            --danger-color: #dc3545;
            --crispr-green: #20c997;
            --crispr-orange: #fd7e14;
            --crispr-purple: #6f42c1;
        }
         
        
        .design-summary {
            display: grid;
            grid-template-columns: repeat(auto-fit, minmax(250px, 1fr));
            gap: 1.5rem;
            margin: 2rem 0;
        }
        
        .design-item {
            background: white;
            border-radius: 8px;
            padding: 1.5rem;
            border: 1px solid var(--border-color);
            box-shadow: 0 2px 4px rgba(0,0,0,0.05);
        }
        
        .design-item .value {
            font-size: 1.5rem;
            font-weight: 700;
            color: var(--crispr-green);
        }
        
        .crispr-info-box {
            background-color: rgba(32, 201, 151, 0.05);
            border-left: 3px solid var(--crispr-green);
            padding: 1rem;
            border-radius: 0 4px 4px 0;
            margin: 1.5rem 0;
        }
        
        .crispr-chart-container {
            position: relative;
            height: 300px;
            width: 100%;
            margin: 1.5rem 0;
        }
        
        .crispr-status-indicator {
            display: inline-block;
            width: 12px;
            height: 12px;
            border-radius: 50%;
            margin-right: 8px;
        }
        
        .status-high {
            background-color: var(--success-color);
        }
        
        .status-medium {
            background-color: var(--warning-color);
        }
        
        .status-low {
            background-color: var(--danger-color);
        }
        
        .crispr-classification {
            padding: 1rem;
            border-radius: 8px;
            margin: 1rem 0;
            border-left: 5px solid;
        }
        
        .efficiency-high {
            background-color: rgba(40, 167, 69, 0.1);
            border-left-color: var(--success-color);
        }
        
        .efficiency-medium {
            background-color: rgba(255, 193, 7, 0.1);
            border-left-color: var(--warning-color);
        }
        
        .efficiency-low {
            background-color: rgba(220, 53, 69, 0.1);
            border-left-color: var(--danger-color);
        }
        
        .section-title {
            font-weight: 600;
            margin-bottom: 1rem;
            color: var(--primary-color);
            padding-bottom: 0.5rem;
            border-bottom: 1px solid var(--border-color);
        }
        
        .guide-rna-sequence {
            font-family: 'Courier New', monospace;
            background-color: #f8f9fa;
            padding: 0.5rem;
            border-radius: 4px;
            margin: 0.5rem 0;
            border: 1px solid #e9ecef;
        }
        
        .pam-sequence {
            color: var(--crispr-orange);
            font-weight: bold;
        }
        
        .guide-list {
            max-height: 400px;
            overflow-y: auto;
        }
        
        .guide-item {
            padding: 1rem;
            border: 1px solid var(--border-color);
            border-radius: 6px;
            margin-bottom: 1rem;
            transition: all 0.3s;
        }
        
        .guide-item:hover {
            box-shadow: 0 4px 8px rgba(0,0,0,0.1);
        }
        
        .guide-item.selected {
            border-color: var(--crispr-green);
            background-color: rgba(32, 201, 151, 0.05);
        }
        
        .guide-score {
            font-size: 1.25rem;
            font-weight: bold;
            color: var(--crispr-green);
        }
        
        .sequence-visualization {
            font-family: 'Courier New', monospace;
            background-color: #f8f9fa;
            padding: 1rem;
            border-radius: 4px;
            margin: 1rem 0;
            border: 1px solid #e9ecef;
            overflow-x: auto;
            white-space: nowrap;
        }
        
        .guide-highlight {
            background-color: rgba(32, 201, 151, 0.3);
            padding: 2px;
            border-radius: 2px;
        }
        
        .pam-highlight {
            background-color: rgba(253, 126, 20, 0.3);
            padding: 2px;
            border-radius: 2px;
        }
        
        .offtarget-site {
            background-color: rgba(220, 53, 69, 0.1);
            padding: 0.5rem;
            border-radius: 4px;
            margin: 0.5rem 0;
            border-left: 3px solid var(--danger-color);
        }
        
        .parameter-control {
            margin-bottom: 1rem;
        }
        
        .slider-value {
            display: inline-block;
            min-width: 40px;
            text-align: right;
        }
        
        .guide-details {
            background-color: white;
            border-radius: 8px;
            padding: 1.5rem;
            border: 1px solid var(--border-color);
            margin-bottom: 1.5rem;
        }
        
        .nav-tabs .nav-link.active {
            color: var(--primary-color);
            font-weight: 600;
            border-bottom: 3px solid var(--primary-color);
        }
        
        .guide-feature {
            display: flex;
            justify-content: space-between;
            padding: 0.5rem 0;
            border-bottom: 1px solid var(--border-color);
        }
        
        .guide-feature:last-child {
            border-bottom: none;
        }
        
        .feature-name {
            font-weight: 500;
        }
        
        .feature-value {
            color: var(--text-muted);
        }
        
        .feature-good {
            color: var(--success-color);
        }
        
        .feature-warning {
            color: var(--warning-color);
        }
        
        .feature-bad {
            color: var(--danger-color);
        }
        
        .badge-guide {
            background-color: var(--crispr-green);
            color: white;
        }
        
        .badge-pam {
            background-color: var(--crispr-orange);
            color: white;
        }
        .form-range {
    width: 100%;
    height: 1.5rem;
    padding: 0;
    -webkit-appearance: none;
    -moz-appearance: none;
    appearance: none;
    background-color: transparent;
}
        .secondary-structure {
            font-family: monospace;
            white-space: pre;
            background-color: #f8f9fa;
            padding: 1rem;
            border-radius: 4px;
            margin: 1rem 0;
            border: 1px solid #e9ecef;
            overflow-x: auto;
        }
        
        .structure-dot {
            color: #007bff;
        }
        
        .structure-parenthesis {
            color: #dc3545;
        }
        
        .structure-energy {
            color: #28a745;
            font-weight: bold;
        }
        
        .progress {
            height: 8px;
        }
        
        .advanced-options {
            background-color: #f8f9fa;
            border-radius: 6px;
            padding: 1rem;
            margin: 1rem 0;
        }
        
        .genome-selector {
            margin-bottom: 1rem;
        }
        
        .batch-input {
            margin-bottom: 1rem;
        }
        
        .export-options {
            margin-top: 1rem;
            padding-top: 1rem;
            border-top: 1px solid var(--border-color);
        }
        
        .history-item {
            padding: 0.75rem;
            border: 1px solid var(--border-color);
            border-radius: 4px;
            margin-bottom: 0.5rem;
            cursor: pointer;
        }
        
        .history-item:hover {
            background-color: var(--light-gray);
        }
        
        .validation-alert {
            margin: 1rem 0;
        }